Biospecimen Pre-analytical Variables (BPV) Program

The Biospecimen Pre-analytical Variables (BPV) Program is a National Cancer Institute-sponsored study to systematically assess the effects of pre-analytical factors on the molecular profile of biospecimens. A robust biospecimen collection infrastructure was established to prospectively collect biospecimens using rigorous standard operating procedures to control for most variables while introducing experimental conditions to study specific biospecimen handling issues, including the cold ischemic time (delay to formalin fixation), time in formalin, freezing methods, as well as storage temperatures and durations. RNA and DNA from biospecimens collected under these conditions was analyzed on multiple molecular platforms. The potential effects of these pre-analytical conditions on protein integrity and detection of metabolites were also examined. Data from this study will be used to develop evidence-based biospecimen standard operating procedures and best practices for fit-for-purpose collection, processing, and storage of biospecimens.

BPV biospecimens are available for research use

The remaining specimens are currently managed through a collaboration between Van Andel Research Institute and NCI. Specimens may be accessed for biospecimen research or clinical assay development upon request. Researchers may also enter into a collaborative research agreement with NCI to extend the BPV studies; please contact us at ncibbrb@nih.gov for more information.